在某网站看到的

oio

最近老在研究国外的生发产品，在美国AMAZON网看到的用户的评价，这是PERFECT HAIR 15%MINOXIDIL的评价：


  rub it in!, February 27, 2010
By  D. Hines (Seattle, WA USA) - See all my reviews
     

Amazon Verified Purchase(What's this?)
I started losing my hair when I was 19. I'm 24 now and have noticeably thinner hair on the entire top of my head. I have used rogaine, scalpmed and ovation cell therapy to try and at least save what I still have and nothing seems to have done little more than just slow down the process.

I ordered the above 15% minoxidil 5% azelaic acid solution above about 2 months ago. Since then I have used it once a day along with ovation cell therapy conditioner. It's been very tough to tell if any progress has been made since any hair regrowth anyone experience takes a great deal of time. Yesterday I decided to shave my head, as I do every couple of months. Upon cutting off my hair I noticed something surprising: there were very noticeably thicker sections in straight lines going across the top of my head! I see this as direct proof of the product working, as I have been applying the solution every day in the exact same pattern. While I look a little funny now with lines of thick and thin hair going over the top of my head, I am extremely happy to have found something that is actually working so far.


Now I just have to be sure to rub it in when I use it so I can hopefully get the same results everywhere! I also love how cheap this product is. This is much easier financially to restock every two months than scalpmed as I was previously using (scalpmed is ~$160 per kit I believe).

Pros:
Seems to actually work! It is very cheap compared to pretty much every alternative. It dries much faster and leaves your hair feeling less greasy than rogaine or scalpmed.

Cons:
The dropper it comes with is sometimes annoying to use as you can never actually fill it to 1mL. I just ordered a second bottle and the new dropper didn't even have the measurements labeled on it. Also, occasionally a white residue will form on your hair where you applied it. I've been fixing this by simply going to the restroom when I notice it and running a damp hand through my hair to get rid of it. Help other customers find the most helpful reviews   
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有没有人用过PERFECT HAIR 15%的米诺，大家说说经验啊

爱在西元前

15%的米诺虽然好 但副作用还是蛮吓人的

春天来啦

写的都是什么啊,看不懂的啊

52012

scalpmed是什么？他是说15%的比5%落健好用，效果还可以。

tyler

没见他提PERFECT HAIR呢。只说了15%米诺5%杜鹃花酸溶液。。

oio

最近我在脱发论坛看到有人提起perfect hair 15% MINOXIDIL,在美国AMAZON的网站，看到购买该产品的用户对使用PERECT HAIR 15%的评价，大家也可取去AMAZON看看。

oio

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    Cautiously Optimistic - First bottle and see some results., March 1, 2010
By  Micah "Micah" (Northern Virginia, USA) - See all my reviews

Amazon Verified Purchase(What's this?)
Years ago used 5% minoxidil (Rogaine) liquid with fair results of stopping loss, but no growth. I have just started (first bottle) with Perfect Hair Solutions 15% Premium with Azelaic acid, and I'm surprised that I can see more small fine hairs on the crown (bald spot) than 3 weeks ago. I feel its a bit too early to be absolutely sure, so I am going to continue with another bottle. Maybe the Azelaic acid is making the difference I don't know. Applies well, feels great. I let it absorb for 1/2 hour before adding hair darkeners, spray etc. Only using for 2 days on and 1 day off (because I don't wash my hair every day). I am 59 years old. Help other customers find the most helpful reviews   
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oio

好像买PERFECT HAIR 15%的试试啊。有用过的吗，大家给点建议

oio

Most Helpful Customer Reviews

   1 of 1 people found the following review helpful:
4.0 out of 5 stars it seems to be working....., December 31, 2009
By  GV (Rio Grande Valley, TX) - See all my reviews

Amazon Verified Purchase(What's this?)
I've been using this for about a month and seems to be working. I've noticed some new thicker hair growth even near my hair line. It is very easy to apply, dries quickly and does not smell nor stains which is a major plus compared to other products. However, it dries my hair and scalp to a point that I cannot apply it and go so I put it on at night and rinse it off in the morning. It would be perfect otherwise although it seems to early to see how effective it really is. Help other customers find the most helpful reviews   
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   5.0 out of 5 stars Awesome Product! Works very fast!, February 6, 2010
By  P. Castillo - See all my reviews
(REAL NAME)     

I used this one for myself instead of my husband and within a few weeks I could notice a difference in the thickness of my hairs and even the feel of it was much better. It was not too greasy at all, so I was still able to sttle my hair the way I always do, and my friends even noticed my hair beginning to grow back, I was so excited I bought the 6 of them. Before that I used the 2%, and that worked pretty good as well. Help other customers find the most helpful reviews   
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   5.0 out of 5 stars excellent product, non-greasy solution, February 6, 2010
By  C. Money (New York, NY) - See all my reviews
(REAL NAME)     

I had my brother try this one out, when I used the 15%. This seems to be working pretty good for him as well. I could see some growth within a 6 weeks which is actually pretty fast. I would recommend starting at this strength and moving up once the growth has slowed down a little. Great product though!!! Help other customers find the most helpful reviews   
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adidasi

晕！我是学俄语的……

牵强

PERFECT HAIR的米诺不是说会有副作用吗

oio

Inhibition of 5a-reductase activity in human skin by zinc and azelaic acid

D. STAMATIADIS, MARIE-CLAIRE BULTEAU-PORTOIS AND IRENE MOWSZOWICZ

Laboratoire de Biochimie B, Hopital Necker-Enfants-Malades, Paris, France

SUMMARY

The effects of zinc sulphate and azelaic acid on 5a-reductase activity in human skin were studied using an in vitro assay with 1,2[3H]-testosterone as substrate. When added at concentrations of 3 or 9 mmol/l, zinc was a potent inhibitor of 5a-reductase activity. At high concentrations, zinc could completely inhibit the enzyme activity. Azelaic acid was also a potent inhibitor of 5a-reductase; inhibition was detectable at concentrations as low as 0.2 mmol/l and was complete at 3 mmol/l. An additive effect of the two inhibitors was observed. Vitamin B6 potentiated the inhibitory effect of zinc, but not of azelaic acid, suggesting that two different mechanisms are involved. When the three substances were added together at very low concentrations which had been shown to be ineffective alone, 90% inhibition of 5a-reductase activity was obtained. If this inhibition is confirmed in vivo, zinc sulphate combined with azelaic acid could be an effective agent in the treatment of androgen related pathology of human skin.



Skin is a target tissue for androgens and as a result increased androgen activity is accompanied by a well-defined skin pathology including hyperseborrhoea, acne and hirsutism or alopecia. In skin, as in many other target tissues, the reduction of testosterone to dihydro-testosterone (DHT) by the enzyme 5a-reductase is the most important of the enzymatic processes involved in androgen activity; indeed DHT is a more potent androgen than is testosterone, due to its greater affinity for the androgen receptor. The active DHT is formed at the target cell site and the enzyme 5a-reductase, therefore, acts as an amplifier of the androgen signal.1 DHT is generally considered responsible for the stimulation of the sebaceous gland2 and increased local formation of DHT has been documented in acne3 and in hirsutism.4 Any product capable of limiting local DHT production could represent, therefore, a potential therapeutic agent. The trace element zinc has been used in dermatology from the times of the Ancient Egyptians; only more recently has it been shown to reduce sebum secretion.5 In addition it is involved in numerous enzyme systems6 and has been reported to play a regulatory role in testosterone metabolism in the human prostate gland.7 We have previously shown that zinc can inhibit, in vitro, the 5a-reductase of human skin.8 Also, topical application of azelaic acid has been reported to have beneficial effects on acne vulgaris.9 In the present study we have examined the in vitro effects of zinc and azelaic acid on testosterone metabolism and in particular, on 5a-reductase activity.

METHODS

Materials

1,2[3H]-Testosterone (specific activity 60 mCi/mmol), [14C]-testosterone (specific activity 60 mCi/mmol), [14C]-dihydrotestosterone (specific activity 58 mCi/mmol) and [14C]-delta 4-andros tenedione (specific activity 57 mCiimmol) were obtained from Amersham, France and purified on celite columns before use. [14C] androstanediols (3 delta and 3 delta Adiols) were prepared by sodium borohydrate reduction of [14C]-DHT as previously described.10 Unlabelled steroids were purchased from Sigma (St. Louis, MO, U.S.A) and crystallized in methanol before use. Stock solutions (10 mmol/l) were prepared in ethanol and stored at 4ºC. NADPH was obtained from Boehringer Mannheim, France S.A. (Meylan) and diluted in buffer before use. All solvents and reagents were of analytical grade. Zinc sulphate, azelaic acid and pyridoxine (vitamin B6) were kindly provided by the Laboratoires Bailleul.

Tissue preparation

Foreskins from normal children (2-3 months old) undergoing circumcision were obtained, with informed consent from the parents, snap frozen in liquid nitrogen and stored at - 80ºC. They were then homogenized with a Polytron homogenizer (five 10s strokes) in ice-cold Krebs-Ringer phosphate buffer (120 mmol/l NaCl, 4.8 mmol/l KCl, 2.6 mmol/l CaCl2, 1.2 mmoI/l MgSO4), pH 6.4 and used as a source of 5a-reductase.

Enzymatic assays

The assay for 5a-reductase activity in human skin homogenates has been reported previously.11 Increasing concentrations of [3H]-testosterone (9, 10, 15, 20, 30, 50, 100 and 500 mmol/l) were evaporated in glass tubes and NADPH (2.4 mmol/1) diluted in buffer was added; the reaction was initiated by the addition of an amount of homogenate corresponding to 10 mg of tissue and the volume adjusted to 1 ml with buffer. Incubations were carried out in a Dubnoff metabolic incubator at 37 C for periods of 5 to 30 min and stopped by the rapid addition of 10 ml of ethyl acetate/cyclohexane 1:1 (v/v). After addition of [14Cl-steroids to monitor recovery and unlabelled tracers for easy visualization, the steroids were extracted and evaporated to dryness. Tubes without homogenates, but containing the same amount of substrate, buffer and cofactors were always incubated in parallel to determine blank values. The metabolites were separated by thin-layer chromatography on silica gel, in chloroform/methanol 97.5:2.5 (v/v) -Testosterone and delta4-androstenedione were visualized under UV light (240 nm) and 5a-reduced steroids using iodine vapour. Steroids were scraped from the plate and eluted in ethanol and acetone. 3 a- and 3 beta-adiols, not separated in this system, were eluted together. Samples were counted in a Packard 300 C liquid scintillation spectrometer with an efficiency of 68 % for [14C] and 30% for [3H]. After correction for recovery and deduction of blank values, 5a-reductase activity was expressed as fmol (DHT + Adiols)/h/mg of tissue.

Effect of zinc and azelaic acid on 5a-reductase activity

Zinc sulphate was added to the incubation tubes at final concentrations of 0.5, 1.5, 3.9 or 15 mmol/l diluted in 100 microl buffer, and the assay was performed as described above. Azelaic acid was added at final concentrations of 0.1, 0.2, 0.5 or 3 mmol/1 diluted in 10 microl ethanol. The same amount of ethanol was also added to the control tubes. In some experiments, pyridoxine(vitamin B6) at a final concentration of 0.025% was also added to the incubation medium. These compounds were added separately or together in various concentrations in order to determine the minimal concentrations which gave a maximal inhibitory effect.

RESULTS

Preliminary experiments have shown that incubation for 5 min gave good estimates of the initial velocity of the reaction for substrate concentrations as low as 10 nmol/l (data not shown). We have used 20 nmol/l testosterone and 5 min incubations to study the effects of zinc and azelaic acid on 5a-reductase activity.

oio

Effect of zinc and azelaic acid On 5a-reductase activity

The effects of increasing concentrations of zinc sulphate (1.5 to 15 mmol/1) and azelaic acid (0.1 to 3 mmol/l) on 5a-reductase activity were studied separately and the results are shown in Figure I. In both cases, there was a dose-dependent inhibition of the enzyme activity; 98% inhibition was observed with 15 mmol/l ZnSO4, and with 3 mmol/l azelaic acid. When the two compounds were added together to the incubation medium at concentrations expected to give 50-60% inhibition (3 mmol/1 and 0-5 mmol/l, respectively), 95% inhibition was observed (data not shown).

Effects of vitamin B6 on zinc and azelaic acid inhibition of 5a-reductase activity

The addition of vitamin B6 (0.025%) to zinc sulphate (1.5 or 3 mmol/l) resulted in a two-fold increase in the inhibition of the enzyme activity (Fig. 2a). In contrast, vitamin B6 had no effect on 5a-reductase activity when added alone or together with azelaic acid (Fig. 2b). These results suggest that zinc and azelaic acid might inhibit 5a-reductase activity through two different mechanisms.
Effect of simultaneous addition of zinc, azelaic and vitamin B6 at low concentrations

The additive effect of the three compounds was studied in order to determine minimal concentrations which would effectively inhibit 5a-reductase activity. From the present results, the concentrations used (0.025% vitamin B6, 0.5 mmol/l ZnSO4, and 0.1 mmol/I azelaic acid) were expected to have a minimal effect, or no effect at all, on the enzyme activity. Vitamin B6 0.025% and azelaic acid at 0.1 mmol/l had no effect on the enzyme activity while ZnS04 at 0. 5 mmol/l gave less than 30% inhibition (Fig. 3). In contrast, when all three compounds were added together at these concentrations, 90% inhibition Of 5a-reductase activity is observed (Fig. 3).

DISCUSSION

Several previous studies have established the inhibitory action of zinc on the 5a-reductase of human prostate.7, 12, 13 We have shown previously that zinc has an inhibitory effect on 5a-reductase in human skin.8 The present study confirms this effect. In addition, since topical azelaic acid has been reported to have beneficial effects in acne vulgaris,14 we studied the effects of azelaic acid on 5a-reductase activity. The results have shown that azelaic acid is a potent in vitro inhibitor of this enzyme in skin homogenates.

The use of in vitro assays to evaluate the local anti-androgenic action of potential 5a-reductase inhibitors has been reported previously in the study of the inhibitory effect of progesterone.15,16 The main interest of these studies is that they enable the distinction to be made between a local and a systemic effect of the inhibitor. They are of particular value when the inhibitor can be applied topically, as is the case with zinc and azelaic acid, and therefore is less likely to exert systemic effects.

The purpose of the present study was to investigate 5a-reductase inhibition with a view to eventual application in physiological or pathological situations. As plasma testosterone levels vary from 2 nmol/I (women) to 20 nmol/l (men), it appeared pertinent to study zinc and azelaic acid inhibition of 5a-reductase activity at this range of substrate concentrations under conditions which allow a precise measurement of enzyme kinetics.

FIGURE 3. Effect of zinc sulphate, azelaic acid (AA) and vitamin B6 (VB6) alone and in combination on 5a-reductase activity in human skin homogenates. Results are expressed as percentages of controls without inhibitor. Values are means of duplicate determinations. [3H] -T = 3H-testosterone.
We have demonstrated a very large inhibition of 5a-reductase activity in the presence of ZnSO4, at concentrations of 3 to 15 mmol/l; 98% inhibition was obtained with the highest concentration. In the human prostate, physiological concentrations of zinc are about 0.2 mmoI/I and high correlation has been found between zinc concentration and 5a-reductase activity.17 However, a biphasic effect of zinc on 5a-reductase activity in the human prostate has been reported, with potentiation at low concentrations (0.1 micro mol/l) and inhibition at higher concentrations (3 to 300 mmol/l).7 This inhibition was shown to be non-competitive relative to testosterone, but competitive relative to NADPH formation. Our preliminary studies (data not shown) seemed to indicate that zinc at low concentrations (0.5 to 3 mmol/l) competitively inhibits 5a-reductase activity while at higher concentrations (3 to 15 mmol/l) it acts as a noncompetitive inhibitor. These results suggest that zinc at different concentrations may act by different mechanisms and that this metal ion interferes with different enzymes, since it also inhibits NADP reduction.

Dicarboxylic acids containing 8 to 13 carbon atoms undergo beta-oxidation and have been shown to be potent inhibitors of oxydoreductases. It has been proposed that azelaic acid could competitively occupy the NADPH-binding site of 5a-reductase thus resulting in inhibition of the enzyme.14 In our experiments, azelaic acid was a potent inhibitor Of 5a-reductase activity. When zinc and azelaic acid were added together, the effect of these two inhibitors was additive suggesting that they may act by two different mechanisms.
Pyridoxine (vitamin B6) is known to interfere with fat metabolism in the skin and, therefore, to play a role, like the androgens, in the regulation of sebum excretion.18 It improves acne lesions in adolescents19 and is more active on topical than on systemic administration.18 This led us to examine the combined effects of vitamin B6 and zinc. Interestingly, whereas vitamin B6 alone had no effect on 5a-reductase activity of human skin it strongly potentiated the inhibitory effect on zinc. In contrast, vitamin B6 did not potentiate the inhibition of 5a-reductase by azelaic acid. This further supports the hypothesis that zinc and azelaic acid act by two different mechanisms.
When the three substances were tested together, 90%, inhibition of the enzyme was obtained at very low concentrations which barely had any effect when tested separately.

If this inhibition is confirmed in vivo, a combination of these substances could provide an effective topical treatment for androgen related pathology of human skin.

ACKNOWLEDGMENTS

This work was supported in part by a grant from the Scientific Council of the Faculty of Medicine Pitie-Salpetriere, University Paris VI, France and by Bailleul Laboratories, Paris, France.
REFERENCES

1 Mauvais-Jarvis P, Kuttenn F, Mowszowicz I. Androgen metabolism in human skin: importance of dihydrotestosterone formation in normal and abnormal target cells. In: Androgenization in Women (Molinatti G, Martini L, James VHT, eds) New York, Raven Press, 1983; 47-63.

2 Takayasu S, Adachi K. The conversion of testosterone to 17 beta-hydroxy- 5a-androstan- 3 -one dihydrotestosterone by human hair follicles. J Clin Endocrinol Metabol 1972; 34: 1098-101.

3 Sansone G, Reisner RM. Differential rates of conversion of testosterone to dihydrotestosterone in acne and in normal human skin: a possible pathogenic factor in acne. J Invest Dermatol 1971; 56: 366-72.

4 Kuttenn F, Mowszowicz I, Schaison G, Mauvais-Jarvis P. Androgen production and skin metabolism in hirsutism. J Endocrinol 1977; 75: 83-91.

5 Demetree JW, Safer LF, Artis WM. The effect of zinc on sebum secretion rate. Acta Dermatovenerol, 1980; 60: 166-9

6 Prasad AS. Clinical, endocrinological and biochemical effects of zinc deficiency. Clinics Endocrinol, Metabol 1985; 14: 567-89.

7 Leake A, Chisholm GD, Habib FK. The effect of zinc on the 5a-reduction of testosterone by the hyperplastic hurnan prostate gland. J Steroid Biochem 1984; 20: 651-5.

8 Starnatiadis D, Bulteau-Portois MC, Mowszowicz I. Effet inhibiteur du Zn sur l'activite 5a-reductase de L humaine. Potentialisation par la vitamine B6. Nouvelles Dermatol 1987; 6: 601-3.

9 Nazzaro-Porro M, Passi S, Picardo M et al. Beneficial effect of 15% azelaic acid cream on acne vulgaris. Br J Dermatol 1983; 109: 45-48.

10 Mowszowicz I, Bardin CW. In vitro androgen metabolism in mouse kidney: high 3 keto-reductase (3-hydroxysteroid dehydrogenase) activity relative to 5a-reductase. Steroids 1974; 23: 793-807.

11 Kuttenn F, Mauvais-Jarvis P. Testosterone 5a-reduction in the skin of normal subjects and of patients with abnormal sex development. Acta Endocrinol 1975; 79: 164-76.

12 Wallace AM, Grant JK. Effects of zinc on androgen metabolism in human hyperplastic prostate. Biochem Soc Trans 1975; 3: 540-2

13 Habib FK. Zinc and the steroid endocrinology of the human prostate gland..J Steroid Biochem 1978; 9: 40

14 Breathnach AS, Nazzaro-Porro M, Passi S. Azelaic acid. Br J Dermatol 1984; 111: 115-20.

15 Mauvais-Jarvis P, Kuttenn F, Baudot N. Inhibition of testosterone conversion to dihydrotestosterone in men treated percutaneously by progesterone. J Clin Endocrinol Metabol 1974; 38: 142-7.

16 Vermorken AJM, Goos CMAA, Roelofs HMJ. A method for the evaluation of the local antiandrogenic action of 5a-reductase inhibitors on human skin. Br J Dermatol 1980; 102: 695-701.

17 Sinquin J, Morfin R, Charles JF, Floch, HH. Testosterone metabolism by homogenates of human prostates with benign hyperplasia: effect of tissue concentrations of zinc, magnesium and copper. J Steroid Biochem 1982; 17: 395-400.

18 Schreiner AW, Slinger W, Hawkins VR, Vilter RW. Seborrheic dermatitis; a local metabolic defect involving pyridoxine. J Lab Clin Med 1952; 40: 121-30.

19 Jollife N, Rosenblum LA, Sawhill J. The effects of pyridoxine (vitamin B6) on persistent adolescent acne..J Invest Dermatol 1942; 5: 143-8.

yuanlailai

楼上的即为怎么都是发的一大段一大段的英文啊,

yuanlailai

都看不明白````

internet

看了半天，看明白了些，大概是说杜鹃花酸对抑制DHT是有效的。

oio

又见到新的评价了，打算再观望1个星期再下手，大家给点意见啊


Cautiously Optimistic - First bottle and see some results., March 1, 2010
By  Micah "Micah" (Northern Virginia, USA) - See all my reviews

Amazon Verified Purchase(What's this?)
Years ago used 5% minoxidil (Rogaine) liquid with fair results of stopping loss, but no growth. I have just started (first bottle) with Perfect Hair Solutions 15% Premium with Azelaic acid, and I'm surprised that I can see more small fine hairs on the crown (bald spot) than 3 weeks ago. I feel its a bit too early to be absolutely sure, so I am going to continue with another bottle. Maybe the Azelaic acid is making the difference I don't know. Applies well, feels great. I let it absorb for 1/2 hour before adding hair darkeners, spray etc. Only using for 2 days on and 1 day off (because I don't wash my hair every day). I am 59 years old. Help other customers find the most helpful reviews   
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重新開始

一大串英文,也看不懂啊.